A prerequisite for NGS is the generation of so-called sequencing libraries out of the correspondent DNA or RNA sample. It consists mainly in the ligation of adaptors, which make the sequencing reaction possible. There are many protocols and kits available depending on sample source and biological question of interest.
The Next Generation Sequencing Core Facility offers two major pipelines:
- DNA preparation - suitable for all types of DNA samples e.g. ChIP, gDNA, cDNA, amplicons
- RNA preparation - applications: sequencing of total RNA, purified mRNA or ribosomal-depleted RNA
Preparation of libraries by user is encouraged and supported.