Preclinical Phenotyping

  • State-of-the-art of phenotypic screening of Mouse Behavior, Metabolism & Physiology.
  • State-of-the-art of phenotypic screening of Mouse Behavior, Metabolism & Physiology.
  • State-of-the-art of phenotypic screening of Mouse Behavior, Metabolism & Physiology.
  • State-of-the-art of phenotypic screening of Mouse Behavior, Metabolism & Physiology.

Phenotyping the Mouse is becoming increasingly important in the complex analysis of genetically manipulated mice. The Preclinical Phenotyping Facility (pcPHENO) at VBCF, Vienna was established in September, 2012. Its primary objective is to provide centralized support and services to VBC investigators developing and studying genetically modified animal models relevant to human diseases.

Services

    Behavioral Tests and Physiological Assays

    Behavioral Tests and Physiological Assays

    First, general health and basic sensori-motor function screening are performed to detect the presence of any early neurological/behavioural abnormalities exhibited by the mouse related to its genotype.

    The first line screening includes:

    • Modified SHIRPA test (protocol)
    • Grip strength (protocol)
    • Open field (protocol)
    • Y maze (protocol)

    Metabolic Studies

    Metabolic Studies

    • Food  and drinking behavior and locomotor activity
    • Food  and drinking behavior and activity with running wheels

    Motor coordination and balance

    Motor coordination and balance

    • Rotarod (protocol)
    • Beam Walking
    • Gaiting - Motorater
    • Swim ability test (protocol)
    • Ladder walking

    For further screening of complex emotional phenotypes, the following tests may be used.

    Anxiety/depression-related behaviour

    Anxiety/depression-related behaviour

    • Elevated plus maze
    • Light/Dark transition box
    • Free exploration
    • Tail suspension test (protocol)
    • Forced swim test

    Advanced tests will be selected based on scored observations and previous tests.

    Learning and Memory

    Learning and Memory

    • MWM task
    • Conditioned place preference test
    • Object recognition test
    • Fear conditioning
    • 3 choice serial reaction time test
    • Active/Passive Avoidance tasks

    Pain studies

    Pain studies

    • Dynamic plantar aesthesiometer
    • Hot/Cold plate sensitivity test

    Social interactions

    Social interactions

    • Aggressive behavior
    • Sexual behaviour
    • Maternal behaviour

    (Neuro)Physiological Analysis

    (Neuro)Physiological Analysis

    DSI telemetry system

    • ECG & temperature and activity (TA11ETA-F10)
    • Blood pressure & temperature and activity (TA11PA-C10)
    • EEG & temperature and activity (TL11M2-F20-EET)

    Resources

    Lab equipment

    Description of test systems

    Coming soon!

    Miscellaneous

    Coming soon!

    User Information

    Getting started

    Investigators wishing to develop a project with the pcPHENO Facility are advised to contact Dr. Sylvia Badurek. The process begins with a discussion of the scientific questions and proceeds to the design of appropriate experimental project. Please note that live animal importation directly into the pcPHENO facility is presently restricted.

    In general, 10 age-matched mice of each gender and of each genotype (–/–, +/– and +/+) are the minimum number of animals that can be submitted in order to obtain meaningful data. The wildtype animals have to be littermates of the mutant animals. The age at which mice are submitted depends upon the age at which the phenotype becomes apparent – if there is no clinical phenotype, submitting a set of young animals (6-8 weeks) is recommended. The researcher should use the smallest number of mice necessary and sufficient to accomplish the research goals, especially in studies which involve manipulations that are potentially detrimental to the animal. The number of animals used in an experiment can often be dramatically reduced by pilot studies, good experimental design and the use of statistical tests that enable several factors to be examined simultaneously.

    Citing the facility

    How should I acknowledge The VBCF pcPHENO Service?

    We all like to receive credit for the work we do don't we?
    Continuation of funding for the VBCF pcPHENO facility by the city of Vienna and the ministry of science depends on documented evidence of contribution to scientific output.
    We therefore demand acknowledgement of utilization of the facility in publishing work.
    A simple statement is sufficient and can either be placed in the materials and methods section or at the end of your manuscript (acknowledgements) depending on journal format.

    Suggested format:
    The XXXXXX was performed by the Preclinical Phenotyping Facility at Vienna Biocenter Core Facilities (VBCF), member of Vienna Biocenter (VBC), Austria.

    In case of (co-)authorship:
    The Vienna Biocenter Core Facilities (VBCF) Preclinical Phenotyping Facility acknowledges funding from the Austrian Federal Ministry of Science, Research & Economy; and the City of Vienna.

    Training

    Coming soon!

    Links

    Publications

    Neuromuscular synapse integrity requires linkage of acetylcholine receptors to postsynaptic intermediate filament networks via rapsyn-plectin 1f complexes.
    Mihailovska E, Raith M, Valencia RG, Fischer I, Banchaabouchi MA, Herbst R, Wiche G.
    Mol Biol Cell. 2014 Oct 15. pii: mbc.E14-06-1174. (abstract)

    Team

    Sylvia Badurek

    Sylvia Badurek

    Core Facility Head
    VBC2 / PG2

    Anna Jelem

    Anna Jelem

    Behavioral Assistant
    VBC2 / PG2

    Klaus Kraitsy

    Klaus Kraitsy

    Behavioral Specialist
    VBC2 / PG2

    Contact