Tagged FlyFos TransgeneOme (fTRG)
The VDRC is very pleased to announce the acquisition of a fantastic new collection of transgenic flies for analysis of gene function and protein localization: the Tagged FlyFos TransgeneOme (fTRG) library.
Full details about the lines can be found in the following publication: A genome-wide resource for the analysis of protein localisation in Drosophila, Sarov et al (2015).
These lines were generously donated to the VDRC by Frank Schnorrer (Max Planck Institute of Biochemistry, Martinsried, Germany) and Vijay Raghavan (National Center for Biological Sciences, TIFR, Bangalore, India) for distribution to the Drosophila research community.
What are the fTRG lines?
The ‘Tagged FlyFos TransgeneOme (fTRG)’ library is comprised of a total of 880 transgenic fly lines, covering 826 different genes. Each line has been engineered to tag a specific protein with a multi-epitope tag at its C-terminus, for use in a variety of downstream applications.
The broad application spectrum includes live imaging, subcellular localization and interaction proteomics of selected gene products at all stages of Drosophila development. Thus the fTRG collection adds considerably to the resources available to Drosophila researchers and their versatility opens up a wide range of previously unfeasible experiments.
How can I get them?
The new collection of lines is available to all registered VDRC users and can be ordered, as for all VDRC lines, by going to www.vdrc.at and following the ordering instructions.
All the stocks can be found on the VDRC homepage (www.vdrc.at ) under the ‘fTRG Stocks’ icon or 'Browse the fTRG Library' and a full list of these lines can be downloaded here:'Download the fTRG Library'
If you would like to see whether there is a tagged line for your gene of interest, use the ‘Quick Search’ tool to search by gene name or CG number. The stocks can then be filtered by library type to list only those in the ‘fTRG’ library.
Clicking on the ‘VDRC ID’ will open a detailed view of that particular line.
How do I acknowledge use of these lines?
Please reference Sarov et al (2015) and acknowledge the VDRC for providing the lines.