Fluorescence-lifetime imaging microscopy (FLIM)

…for measuring fast fluorescence dynamics:

The FLIM microscope is a confocal microscope that allows one to, among other things:

1. Quantitatively measures the concentration of proteins and how fast they diffuse inside a living cell.
2. Spatially map the decay rate of fluorophore’s with picosecond resolution (fluorescence lifetime) and derive distances between labelled proteins with sub-nanometer resolution (FRET).

Lattice light-sheet microscope (LLS)

…for fast 3D high resolution imaging of sub-cellular and cellular processes:

The LLS is a novel light sheet microscope first developed by 2014 Nobel laureate Eric Betzig.

It is capable of imaging intra-cellular processes with very high spatio-temporal resolution and minimal phototoxicity, allowing one to study the fast dynamics of life’s fundamental processes in 3D.

Brillouin Light Scattering Microscope (BLSM)

…for ultra-high resolution spectral imaging of a sample to deduce mechanical and structural properties:

The BLSM is a microspectroscopy setup that allows for the 3-dimensional label-free mapping of the viscoelastic properties within live cells and tissue, with near diffraction limited resolution.

Single Plane Illumination Microscope (LSPIM)

…for fast 3D high resolution imaging deep inside tissue:

The L-SPIM is a light sheet microscope optimised for high resolution imaging of plant root growth and high-speed volume imaging of neural activity in C. elegans.